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Components of Dentinal Adhesives Modulate Heat Shock Protein 72 Expression in Heat-stressed THP-1 Human Monocytes at Sublethal Concentrations

¹ Department of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University Graduate School of Dental Medicine, Kita 13, Nishi 7, Kita-ku, Sapporo 060-8586, Japan;
² Department of Oral Rehabilitation, Medical College of Georgia, Augusta, GA 30912-1260; and
³ Department of Oral Functional Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan;

View larger version (24K): [in a new window]   Figure 1. SDH activity of THP-1 monocytes exposed to HEMA and TEGDMA for 7 hrs and measured by the MTT method, with (open circles) and without (solid circles) heat stress at 43°C for 1 hr. Upper-case letters indicate statistical comparisons between concentrations within the no-heat group (ANOVA, Tukey, = 0.05). Lower-case letters show statistical comparisons among the heat-stressed groups. Heat-stressed vs. unstressed controls at the highest concentrations of HEMA and TEGDMA exposure were statistically different (two-tailed t test, p = 10-10 for HEMA, 10-7 for TEGDMA).

View larger version (81K): [in a new window]   Figure 2. HSP72 expression shown in a typical SDS-PAGE gel and Western blotting for TEGDMA with and without 1 hr of heat stress and recovery for 2, 4, or 6 hrs. The expression of HSP72 with silver stain is denoted on the gel (arrow), and the Western blot of HSP72 is shown at the bottom.

View larger version (35K): [in a new window]   Figure 3. HSP72 expression after HEMA and TEGDMA exposure as a percentage of the six-hour, no-resin controls (arbitrarily set to 100%). Cells were heat-stressed for 1 hr, followed by 2, 4, or 6 hrs of recovery. The letter ‘D‘ indicates a statistical comparison among the heat-stressed groups with six-hour recovery and various resin concentrations. Lower-case letters denote a statistical difference from the no-resin (upper-case) control (ANOVA, Dunnett's, = 0.05). The letter ‘C’ compares the four-hour recovery groups at various resin concentrations, whereas ‘B‘ compares two-hour recovery, and ‘A‘ compares the no-heat-stress groups.