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Experimentally Induced Unilateral Tooth Loss: Histochemical Studies of the Temporomandibular Joint

Q. Huang1, D. Opstelten2, N. Samman1,*, and H. Tideman1

1 Department of Oral & Maxillofacial Surgery, Faculty of Dentistry, Prince Philip Dental Hospital, 34 Hospital Road, and
2 Department of Biochemistry, Faculty of Medicine, The University of Hong Kong, Hong Kong;



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Figure 1. Photomicrographs of sections of mandibular condyle stained with hematoxylin and eosin. (A) Normal rabbit; (B) non-functional side of TMJ 3 wks after unilateral removal of teeth; (C) functional side of TMJ 3 wks after unilateral removal of teeth; (D) non-functional side of TMJ 6 wks after unilateral removal of teeth; and (E) functional side of TMJ 6 wks after unilateral removal of teeth. F, fibrous layer; PC, pre-chondroblast layer; FC, functional chondroblast layer; and HC, hypertrophic chondroblast layer. Arrows 1 & 2 = irregular nuclei; arrows 3 & 4 = round and oval nuclei. Bar = 40 µm.

 


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Figure 2. Photomicrographs of sections of mandibular condyle stained with safranin O. (A) Normal rabbit; (B) non-functional side of TMJ 3 wks after unilateral removal of teeth; (C) functional side of TMJ 3 wks after unilateral removal of teeth; (D) non-functional side of TMJ 6 wks after unilateral removal of teeth; and (E) functional side of TMJ 6 wks after unilateral removal of teeth. F, fibrous layer; PC, pre-chondroblast layer; FC, functional chondroblast layer; and HC, hypertrophic chondroblast layer. Arrows: safranin O staining with an intensity obviously higher than normal. Bar = 80 µm.

 





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