Molecular and Cultural Analysis of the Microflora Associated with Endodontic Infections
M.A. Munson1,
T. Pitt-Ford2,
B. Chong2,
A. Weightman3, and
W.G. Wade1,*
1 Department of Microbiology and
2 Department of Conservative Dentistry, Guy’s, King’s, and St. Thomas’ Dental Institute, Guy’s Tower, Guy’s Hospital, King’s College London, London SE1 9RT, UK; and
3 Cardiff School of Biosciences, Cardiff University, Cardiff, UK;
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Figure 1. Proportion of the microflora, categorized by phylum, recovered by molecular and cultural analyses.
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Figure 2. Phylogenetic tree based on 16S rRNA gene sequence comparisons over 1237 aligned bases showing novel phylotypes within the phylum Bacteroidetes. Tree was constructed by the neighbor-joining method following distance analysis of aligned sequences and was rooted for Lewinella persica. Numbers represent bootstrap values for each branch based on data for 100 trees. Accession numbers for 16S rRNA sequences are given for each strain. Scale bar shows number of nucleotide substitutions per site.
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Figure 3. Phylogenetic tree based on 16S rRNA gene sequence comparisons over 1131 aligned bases showing novel phylotypes within the phylum Firmicutes. Tree was constructed by the neighbor-joining method following distance analysis of aligned sequences and was rooted for Atopobium rimae. Numbers represent bootstrap values for each branch based on data for 100 trees. Accession numbers for 16S rRNA sequences are given for each strain. Scale bar shows number of nucleotide substitutions per site.
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