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Microarray Analysis of Gene Expression Changes in Aging in Mouse Submandibular Gland

¹ Department of Biochemistry,
² Department of Oral Surgery, Nihon University School of Dentistry at Matsudo, Nishi 2-870-1 Sakae-cho, Matsudo, Chiba 271-8587, Japan; and
³ Department of Pharmacology, Nihon University School of Medicine, 30 Oyaguchi, Kami-machi Itabashi, Tokyo 173, Japan;

View larger version (44K): [in a new window]   Figure 1. Scatter plot analysis of 1,328 genes. (A) Genes whose expression ratio (2M/15M) was over two-fold changed, indicating increased genes by aging. (B) Genes whose expression ratio (15M/2M) was over two-fold changed, indicating increased genes by aging.

View larger version (38K): [in a new window]   Figure 2. RT-PCR analysis. Total RNAs from two-month-old (2M), 15-month-old (15M), and 23-month-old (23M) were reverse-transcribed. The resulting cDNAs were amplified with gene-specific primers. AA038677, Ca(2+)-transporting ATPase; U49393, sarcoendoplasmic reticulum Ca(2+) ATPase; AA024297, translocating chain-associating membrane protein; W62742, sec 61 alpha subunit; and X03672, mouse beta-actin, as a negative control. Each predicted PCR size is indicated.