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Expression of MMP-8 and MMP-13 mRNAs in Rat Periodontium during Tooth Eruption

¹ Division of Periodontics and Endodontics, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan;
² Division of Oral Molecular Biology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan; and
³ Division of Orthodontics, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan;

View larger version (72K): [in a new window]   Figure 1. Oligonucleotide primers used for Section-RT-PCR (upper). Section-RT-PCR of rat maxillary periodontium in weeks 3 (W3) and 4 (W4) (lower). Gene expression of MMP-8, MMP-13, and GAPDH is identified. Negative controls (non-RT) do not produce bands visible on gels. Positive controls (total RNA of embryonic rat limbs) show the corresponding bands.

View larger version (183K): [in a new window]   Figure 2. Oligonucleotide primers to prepare for RNA probes (upper). RNA polymerases and restriction enzymes used for RNA probes (middle). In situ hybridization for COL I (a-d) and COL III (e-h) during root formation and eruption of maxillary first molars (lower). (a,e) Week 2. (b,c,f,g) Week 4. (d,h) Week 6. Arrowheads in (c) and (g) indicate hybridization signals. B, alveolar bone; P, periodontal ligaments; C, cementum; D, dentin. Bars = 10 µm (a,e); 20 µm (b,d,f,h); 5 µm (c,g).

View larger version (138K): [in a new window]   Figure 3. In situ hybridization for MMP 8 (a-d) and MMP 13 (e-h) during root formation and eruption of maxillary first molars. (a,e) Week 2. (b,c,f,g) Week 4. (d,h) Week 6. The insert in (f) is an image with a higher magnification of the enclosed region. Arrowheads in (c), (e) and (f) indicate hybridization signals. B, alveolar bone; P, periodontal ligaments; C, cementum; D, dentin. Bars = 10 µm (a,e); 20 µm (b,d,f,h); 5 µm (c,g).