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Journal of Dental Research, Vol. 81, No. 1, 43-47 (2002)
DOI: 10.1177/154405910208100110

Plasma Membrane Disruption in Orthodontic Tooth Movement in Rats

M.F. Orellana1, A.K. Smith1, J.L. Waller2, E. DeLeon, Jr.3 and J.L. Borke*,1

1 Department of Oral Biology and Maxillofacial Pathology,
2 Office of Biostatistics, and
3 Department of Orthodontics, Medical College of Georgia, School of Dentistry, Augusta, GA 30912-1129, USA;


Figure 1
Figure 1
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  		Figure 1. Orthodontic mechanics used in this study. (A) Schematic representation of orthodontic appliance for lateral movement of first upper molars. (B) Occlusal view of the activated appliance set on the rat maxilla. The initial expansional force was adjusted to 50 g.

 

Figure 2
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  		Figure 2. Western blot analysis demonstrating the specificity of the sheep anti-rat albumin antibody. Immunostaining of rat maxillary soft tissue homogenate (lane T) and rat plasma (lane P) shows single bands corresponding to the molecular weight of albumin. Lane M contains biotinylated marker proteins.

 

Figure 3
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  		Figure 3. Immunohistochemistry of rat maxillary first molar root tips demonstrating cellular uptake of albumin. (A) Negative control section showing PDL stained by immunohistochemistry without anti-albumin antibody (x25). (B & C) Higher-magnification (x100) micrographs of buccal and lingual areas (respectively) from the same PDL seen in (A), showing no background staining. (D) Tissue section showing immunolocalization of albumin in the PDL from a tooth after 5 min of loading and 2 hrs of recovery (x25). (E & F) Higher-magnification (x100) micrographs of buccal and lingual areas (respectively) from the same PDL seen in (D), showing intracellular localization of albumin. (G) Tissue section from a control rat where teeth were not loaded, showing no apparent staining of the PDL (x25). This section was stained by immunohistochemistry with anti-albumin antibody. (H & I) Higher-magnification (x100) micrographs of buccal and lingual areas (respectively) from the same PDL seen in (G), showing no intracellular or background staining.

 

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