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Mechanotransducers in Rat Pulpal Afferents

¹ Program in Neuroscience, University of Maryland, Baltimore, MD 21201, USA;
² Department of Oral Biology, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, 185 South Orange Ave, Newark, NJ 07103, USA; and
³ Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Pittsburgh, 3500 Terrace Street, Rm E1440 BST, Pittsburgh, PA 15213, USA

Correspondence: ^* corresponding author, goldm{at}dom.pitt.edu

The hydrodynamic theory suggests that pain associated with stimulation of a sensitive tooth ultimately involves mechanotransduction as a consequence of fluid movement within exposed dentinal tubules. To determine whether putative mechanotransducers could underlie mechanotransduction in pulpal afferents, we used a single-cell PCR approach to screen retrogradely labeled pulpal afferents. The presence of mRNA encoding BNC-1, ASIC3, TRPV4, TRPA1, the , β, and subunits of ENaC, and the two pore K⁺ channels (TREK1, TREK2) and TRAAK were screened in pulpal neurons from rats with and without pulpal inflammation. ASIC3, TRPA1, TREK1, and TREK2 were present in ~67%, 64%, 14%, and 10% of pulpal neurons, respectively. There was no detectable influence of inflammation on the proportion of neurons expressing these mechanotransducers. Given that the majority of pulpal afferents express ASIC3 and TRPA1, our results raise the possibility that these channels may be novel targets for the treatment of dentin sensitivity.

Key Words: dentin hypersensitivity • single-cell PCR • retrograde labeling • primary afferent

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