This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Scopus Google Scholar Articles by Palanisamy, V. Articles by Wong, D.T. PubMed PubMed Citation Social Bookmarking                   What's this?

AUF1 and HuR Proteins Stabilize Interleukin-8 mRNA in Human Saliva

¹ School of Dentistry and Dental Research Institute,
² Jonsson Comprehensive Cancer Center,
³ Molecular Biology Institute,
⁴ Division of Head & Neck Surgery/Otolaryngology, School of Medicine, and
⁵ Henry Samueli School of Engineering and Applied Science, University of California Los Angeles, Los Angeles, CA 90095-1668, USA

Correspondence: ^* corresponding author, dtww{at}ucla.edu

Human saliva contains thousands of mRNAs, some of which have translational value as diagnostic markers for human diseases. We have found that more than 30% of the mRNAs detected in human saliva contain AU-rich elements (ARE) in their 3' untranslated regions (3'UTR). Since AREs are known to contribute to RNA turnover by forming complexes with ARE-binding proteins, we hypothesized that salivary mRNA stability is mediated by ARE-binding proteins in human saliva. To test this hypothesis, we monitored the in vitro degradation of a radiolabeled ARE-containing salivary mRNA (IL-8) in salivary protein extracts. The degradation of IL-8 mRNA was accelerated by competition for saliva ARE-binding proteins through the addition of excess unlabeled IL-8 mRNA fragments containing 4 tandem AREs. UV cross-linking and immunoprecipitation experiments revealed 2 ARE-binding proteins, AUF1 and HuR, associated with IL-8 mRNA in saliva. These results demonstrate that ARE-binding proteins contribute to the stability of ARE mRNAs in human saliva.

Key Words: Human saliva • mRNA stability • AU-rich elements • AUF1 • HuR

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Mazan-Mamczarz, Y. Kuwano, M. Zhan, E. J. White, J. L. Martindale, A. Lal, and M. Gorospe Identification of a signature motif in target mRNAs of RNA-binding protein AUF1 Nucleic Acids Res., January 1, 2009; 37(1): 204 - 214. [Abstract] [Full Text] [PDF]