Journal of Dental Research

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Hattori, N.
Right arrow Articles by Noguchi, T.
PubMed
Right arrow PubMed Citation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Dental Research, Vol. 87, No. 12, 1117-1121 (2008)
DOI: 10.1177/154405910808701214

Biomaterials & Bioengineering

Methyl Methacrylate Activates the Gsta1 Promoter

N. Hattori1,*, T. Suzuki2, S. Jinno1, H. Okeya1, A. Ishikawa3, C. Kondo2, T. Hayashi4, M. Ito1, T. Kanamori2, T. Kawai4 and T. Noguchi1,*

1 Department of Periodontology,
2 Department of Biochemistry,
3 The Second Department of Prosthodontics, and
4 Department of Dental Material Science, School of Dentistry, Aichi Gakuin University, 1–100 Kusumoto-cho, Chikusa-ku, Nagoya 464–8650, Japan

Correspondence: * corresponding author, srp-psho{at}dpc.agu.ac.jp

Residual monomers in resin-based biomaterials cause cytotoxicity. We previously showed that methyl methacrylate (MMA) induced mRNA expression of the glutathione S-transferase alpha 1 gene (Gsta1) located downstream of the cis-acting anti-oxidant responsive element (ARE). Herein, we tested the hypothesis that MMA activated the Gsta1 promoter through the ARE. HepG2 cells were transfected with a luciferase reporter vector containing the ARE and the Gsta1 promoter (–990 to +46 bp) and cultured for 12 hrs with MMA (initial concentration, 10 mM). Analysis of the expressed luciferase activity indicated that MMA activated the promoter 2.6-fold. MMA (from 1 to 30 mM) dose-dependently increased the promoter activity, which reached a plateau between 6 and 12 hrs. In HepG2 cells transfected with a reporter vector containing 2 AREs and a TATA-like promoter, 10 mM MMA increased the reporter expression 2.8-fold. These results suggest that MMA increases Gsta1 transcription through ARE-mediated promoter activation.

Key Words: methyl methacrylate • glutathione S-transferase • anti-oxidant responsive element • reactive oxygen species • luciferase reporter assay

Abbreviations: ANOVA, analysis of variance • ARE, anti-oxidant responsive element • Gsta1, mouse glutathione S-transferase alpha 1 gene • GSH, glutathione • GST, glutathione S-transferase • Keap1, Kelch ECH associating protein 1 • MMA, methyl methacrylate • NQO1, NAD(P)H:quinone oxidoreductase 1 • Nrf2, nuclear factor erythroid 2-related factor 2 • PCR, polymerase chain-reaction • ROS, reactive oxygen species • SEM, standard error of mean • t-BHQ, tert-butylhydroquinone.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?