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Journal of Dental Research, Vol. 86, No. 9, 878-882 (2007)
DOI: 10.1177/154405910708600914

Biological

Hemoglobin and LPS Act in Synergy to Amplify the Inflammatory Response

C. Bodet, F. Chandad and D. Grenier*

Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada G1K 7P4

Correspondence: * corresponding author, Daniel.Grenier{at}greb.ulaval.ca

Vascular disruption and bleeding during periodontitis likely increase the levels of hemoglobin in gingival crevicular fluid. The aim of this study was to investigate the effect of hemoglobin on the inflammatory responses of human macrophages stimulated with lipopolysaccharides (LPS) isolated from periodontopathogens. The production of interleukin-1 beta (IL-1β), IL-6, IL-8, and tumor necrosis factor alpha (TNF-{alpha}) by macrophages following challenges with Porphyromonas gingivalis and Fusobacterium nucleatum LPS in the presence or absence of human hemoglobin was analyzed by ELISA. The effect of hemoglobin on LPS-binding to macrophages was evaluated with 3H-LPS. Hemoglobin and LPS from periodontopathogens acted in synergy to stimulate the production of high levels of IL-1β, IL-6, IL-8, and TNF-{alpha} by macrophages. Hemoglobin also enhanced LPS-binding to macrophages. This study suggests that hemoglobin contributes to increases in the levels of pro-inflammatory mediators in periodontal sites by acting in synergy with LPS from periodontopathogens, thus favoring the progression of periodontitis.

Key Words: cytokine • Fusobacterium nucleatum • periodontitis • Porphyromonas gingivalis


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S.-i. Tanabe, M. Gottschalk, and D. Grenier
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Innate Immunity, December 1, 2008; 14(6): 357 - 363.
[Abstract] [PDF]