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IL-10 Gene Transfer Attenuates P. gingivalis-induced Inflammation

¹ Department of Periodontology,
² Department of Oral Rehabilitation, and *Department of Prosthodontics, Faculty of Dental Medicine, Hebrew University-Hadassah Faculty of Dental Medicine, PO Box 12272, Jerusalem 91120, Israel

^* corresponding author, mhouri{at}cc.huji.ac.il

IL-10 is an anti-inflammatory cytokine secreted by stimulated Th2 lymphocytes that can down-regulate inflammatory responses to bacterial challenge. We hypothesized that local delivery of IL-10 using gene-transfer will down-regulate inflammatory responses. We examined the effect of IL-10 plasmid injection on the local cytokine response. Two weeks after the implantation of chambers, either IL-10 plasmid or vector was injected into the mice. Four days later, they were challenged with an intra-chamber injection of P. gingivalis. The intra-chamber levels of IL-10, IFN, TNF, and IL-1ß were evaluated after 2 and 24 hrs. The results showed that local IL-10 gene delivery elevated the levels of IL-10 at both time periods. It attenuated the levels of IFN (656 ± 154 to 218 ± 144 pg/mL) and TNF (23 ± 2.0 to 12.5 ± 2.9 ng/mL) at 2 hrs, and of IL-1ß (21.5 ± 5.7 to 12.4 ± 3.0 ng/mL) at 24 hrs. The results suggest the possibility of modulating the local inflammatory response to P. gingivalis by direct IL-10 gene transfer.

KEY WORDS: Porphyromonas gingivalis • gene transfer • inflammation