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Effect of Aqueous Ozone on the NF-B System

¹ Department of Restorative Dentistry & Periodontology,
³ Department of Orthodontics,
⁴ Experimental Surgery & Regenerative Medicine, Department of Surgery, Ludwig-Maximilians-University, Goethe Street 70, 80336 Munich, Germany;
² Institute of Clinical Chemistry & Pathobiochemistry, Klinikum rechts der Isar, Technische Universität München, Germany; and
⁵ Institute of Clinical Chemistry, Medizinische Hochschule Hannover, Germany

^* corresponding author, khuth{at}dent.med.uni-muenchen.de

Ozone has been proposed as an alternative oral antiseptic in dentistry, due to its antimicrobial power reported for gaseous and aqueous forms, the latter showing a high biocompatibility with mammalian cells. New therapeutic strategies for the treatment of periodontal disease and apical periodontitis should consider not only antibacterial effects, but also their influence on the host immune response. Therefore, our aim was to investigate the effect of aqueous ozone on the NF-B system, a paradigm for inflammation-associated signaling/transcription. We showed that NF-B activity in oral cells stimulated with TNF, and in periodontal ligament tissue from root surfaces of periodontally damaged teeth, was inhibited following incubation with ozonized medium. Under this treatment, IB proteolysis, cytokine expression, and B-dependent transcription were prevented. Specific ozonized amino acids were shown to represent major inhibitory components of ozonized medium. In summary, our study establishes a condition under which aqueous ozone exerts inhibitory effects on the NF-B system, suggesting that it has an anti-inflammatory capacity.

KEY WORDS: aqueous ozone • NF-B • signaling • inflammation • oral