HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hahn, C.-L. Articles by Tew, J.G. Search for Related Content PubMed PubMed Citation Articles by Hahn, C.-L. Articles by Tew, J.G.

Rapid Tissue Factor Induction by Oral Streptococci and Monocyte-IL-1ß

¹ Department of Endodontics, School of Dentistry, Lyons Building, Rm. 441, 520 N. 12th Street, PO Box 980566, Richmond, VA 23298-0566, USA;
² Department of Biostatistics and
³ Department of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA, USA

^* corresponding author, tew{at}hsc.vcu.edu

The ability of pro-inflammatory cytokines to promote coagulation prompted the hypothesis that pro-inflammatory cytokines induced by oral streptococci might play a role in the pathogenesis of viridans endocarditis. We used supernatant fluids from peripheral blood mononuclear monocyte (PBMC) cultures, stimulated for just 4–6 hrs with representative streptococcal isolates, to study cytokines that promoted endothelial tissue factor (TF) activity. Neutralizing antibodies demonstrated that interleukin-1ß (IL-1ß) was a major early endothelial TF inducer, and that recombinant IL-1ß was comparable with the supernatant fluid in activity. IL-1ß-rich supernatant fluids from oral streptococci-stimulated or lipopolysaccharide-stimulated PBMC cultures up-regulated the expression of endothelial ICAM-1 and E-selectin. These molecules could help trap TF-producing monocytes or dendritic cells bearing streptococci at the site. Thus, the rapid IL-1ß-inducing capacity of oral streptococci could facilitate the early deposition of bacteria in fibrin clots and promote endocarditis.

KEY WORDS: streptococcus • monocytes • endothelium • tissue factor • IL-1ß