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Regulation of Cementoblast Function by P. gingivalis Lipopolysaccharide via TLR2

¹ Department of Periodontics, School of Dentistry, University of Washington, D322-Health Science Center Box 356365, Seattle, WA 98195-6365, USA;
² Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan; and
³ FBDC-Curso de Odontologia, Brazil

^* corresponding author, somerman{at}u.washington.edu

Although cementoblasts express Toll-like receptors (TLR)-2 and -4, little is known regarding the possible participation of cementoblasts in the inflammatory response. We investigated the effects of Porphyromonas gingivalis lipopolysaccharide (LPS), tetra- and penta-acylated lipid A species (designated PgLPS_1435/1449 and PgLPS₁₆₉₀, respectively), on gene expression of osteoclastogenesis-associated molecules in murine cementoblasts. Real-time quantitative RT-PCR analysis revealed that receptor activator of NF-B ligand (RANKL), interleukin-6, Regulated on activation, normal T-cell expressed, and secreted (RANTES), macrophage inflammatory protein-1, and monocyte chemoattractant protein-1 were rapidly and dramatically induced upon stimulation with PgLPS₁₆₉₀, but only slightly induced with PgLPS_1435/1449. Osteoprotegerin, which was expressed constitutively, was not altered significantly. ELISA demonstrated synthesis of corresponding proteins. PgLPS₁₆₉₀ significantly induced transcripts for NF-B, and this activation was inhibited by pre-treatment with anti-TLR-2 but not with TLR-4 antibodies. These results suggest that cementoblasts participate in the recruitment of osteoclastic precursor cells by up-regulation of chemokines/cytokines.

KEY WORDS: cementoblast • osteoclastogenesis • Toll-like receptor • lipopolysaccharide • Porphyromonas gingivalis