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Toll-like Receptors, NOD1, and NOD2 in Oral Epithelial Cells

¹ Division of Oral Diagnosis, Department of Oral Medicine and Surgery, and
² Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan;
³ Department of Chemistry, Graduate School of Science, Osaka University, Japan; and
⁴ Division of Control of Oral Infection, Hokkaido University Graduate School of Dentistry, Sapporo, Japan

^* corresponding authors, dent-ht{at}mail.tains.tohoku.ac.jp, kyoro{at}mail.tains.tohoku.ac.jp

Oral epithelium might be the first barrier against oral bacteria in periodontal tissue. We hypothesized that oral epithelium is endowed with innate immune receptors for bacterial components, which play roles in host defense against bacterial infection without being accompanied by excessive inflammatory responses. We found clear expression of Toll-like receptor (TLR)4 as well as TLR2, and strong expression of NOD1 and NOD2 in normal oral epithelial tissues by immunohistochemical analysis. We also showed that primary oral epithelial cells in culture expressed these molecules using PCR, flow cytometry, and immunostaining. In inflamed oral epithelium, cell-surface localizations of TLR2 and TLR4 were more clearly observed than in healthy tissue. Upon stimulation with synthetic ligands for these receptors, the expression of ß-defensin 2 was markedly up-regulated. These findings indicate that these molecules in oral epithelial cells are functional receptors that induce antibacterial responses.

KEY WORDS: TLR • NOD • epithelium • ß-defensin 2 • inflammation

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