JDR JDR Most Read Articles
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zavarella, M.M.
Right arrow Articles by Walters, J.D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zavarella, M.M.
Right arrow Articles by Walters, J.D.
J Dent Res 85(5):452-456, 2006
© 2006 International and American Associations for Dental Research

RESEARCH REPORT
Biological

Accumulation of Non-steroidal Anti-inflammatory Drugs by Gingival Fibroblasts

M.M. Zavarella1, O. Gbemi2, and J.D. Walters1,*

1 Sections of Periodontology and
2 Oral Biology, College of Dentistry, The Ohio State University Health Sciences Center, 305 West 12th Avenue, P.O. Box 182357, Columbus, OH 43218-2357, USA

* corresponding author, walters.2{at}osu.edu

Non-steroidal anti-inflammatory drugs (NSAIDs) are used to manage pain and inflammatory disorders. We hypothesized that gingival fibroblasts actively accumulate NSAIDs and enhance their levels in gingival connective tissue. Using fluorescence to monitor NSAID transport, we demonstrated that cultured gingival fibroblasts transport naproxen in a saturable, temperature-dependent manner with a Km of 127 µg/mL and a Vmax of 1.42 ng/min/µg protein. At steady state, the intracellular/extracellular concentration ratio was 1.9 for naproxen and 7.2 for ibuprofen. Naproxen transport was most efficient at neutral pH and was significantly enhanced upon cell treatment with TNF-{alpha}. In humans, systemically administered naproxen attained steady-state levels of 61.9 µg/mL in blood and 9.4 µg/g in healthy gingival connective tissue, while ibuprofen attained levels of 2.3 µg/mL and 1.5 µg/g, respectively. Thus, gingival fibroblasts possess transporters for NSAIDs that are up-regulated by an inflammatory mediator, but there is no evidence that they contribute to elevated NSAID levels in healthy gingiva.

KEY WORDS: naproxen • ibuprofen • analgesic • inflammatory periodontitis






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
IADR Journals Advances in Dental Research ®
Journal of Dental Research ® Critical Reviews (1990-2004)
Copyright © 2006 Institutional Access Guidelines