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Edema in Oral Mucosa after LPS or Cytokine Exposure

Institute of Biomedicine, Section for Physiology, Faculty of Medicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway

^* corresponding author, Nancy.Bletsa{at}biomed.uib.no

Lowering of interstitial fluid pressure (P_if) is an important factor that explains the rapid edema formation in acute inflammation in loose connective tissues. Lipopolysaccharide (LPS) and the pro-inflammatory cytokines interleukin-1beta (IL-1ß) and tumor necrosis factor-alpha (TNF-) are pathogenetic in gingivitis. To test if these substances induce lowering of P_if in rat oral mucosa, we measured P_if with a micropuncture technique. IL-1ß and TNF- caused lowering of P_if, whereas LPS induced an immediate increase in P_if, followed by lowering after 40 min. Measurements of fluid volume distribution showed a significant change in interstitial fluid volume (V_i) 1.5 hr after LPS exposure as V_i changed from 0.41 ± 0.02 to 0.51 ± 0.03 mL/g wet weight (p < 0.05), confirming edema. These findings show that LPS, IL-1ß, and TNF- induce lowering of P_if in the rat oral mucosa and contribute to edema formation in LPS-induced gingivitis.

KEY WORDS: IL-1ß • TNF- • interstitial fluid pressure • micropuncture • inflammation