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J Dent Res 85(3):251-256, 2006
© 2006 International and American Associations for Dental Research

RESEARCH REPORT
Biological

Functional {alpha}1-Adrenoceptor Subtypes in Human Submandibular Glands

Z. Huang1, L.L. Wu2,*, Y.Y. Zhang3, Y. Gao4, and G.Y. Yu1,*

1 Department of Oral and Maxillofacial Surgery and
4 Department of Oral Pathology, Peking University School and Hospital of Stomatology, Zhong Guan Cun South St. 22, 100081 Beijing, PRC;
2 Department of Pathophysiology, Peking University Health Science Center; and
3 Institute of Vascular Medicine, Peking University Third Hospital, Beijing, PRC

* co-corresponding authors, gyyu{at}263.net and pathophy{at}bjmu.edu.cn

{alpha}1-Adrenoceptor has been discovered to exist in many human tissues and mediates important physiological functions. The purpose of this study was to detect the expression, distribution, and function of {alpha}1-adrenoceptor subtypes in human submandibular glands. {alpha}1A- and {alpha}1B-Adrenoceptor mRNAs were identified by reverse-transcription/polymerase chain-reaction (RT-PCR), and their proteins were detected by Western blotting. No expression of the {alpha}1D-adrenoceptor mRNA and protein was found. By in situ hybridization and immunohistochemistry, {alpha}1A- and {alpha}1B-adrenoceptor mRNAs and proteins were shown to be widespread in both ductal and acinar cells. By confocal microscopy, phenylephrine (stimulating both {alpha}1A- and {alpha}1B-adrenoceptors) or A61603 ({alpha}1A-selective agonist) induced an increase in intracellular calcium by 2.33 ± 0.18-fold and 1.81 ± 0.43-fold, respectively, while 5-methylurapidil ({alpha}1A-selective antagonist) partly blocked calcium mobility stimulated by phenylephrine. The results indicated that functional {alpha}1A- and {alpha}1B-adrenoceptors were expressed in human submandibular glands, and might contribute to the regulation of saliva synthesis and secretion.

KEY WORDS: {alpha}1-adrenoceptor • receptor subtypes • neurotransmitter • submandibular gland • tissue distribution






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