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Reduced Antigenicity of Type I Collagen and Proteoglycans in Sclerotic Dentin

¹ Department of SAU & FAL, University of Bologna, Italy;
² Department of Dental Science, University of Bologna, Italy;
³ Department of Oral Biology, School of Dentistry Medical College of Georgia, Augusta, GA, USA; and
⁴ Department of MUN, UCO of Dental Sciences, University of Trieste, Via Stuparich, 1, I-34129 Trieste, Italy

^* corresponding author, lbreschi{at}units.it

Antigenic alterations to the dentin organic matrix may be detected by an immunohistochemical approach. We hypothesized that alterations in the antigenicity of type I collagen and proteoglycans occur in sclerotic dentin under caries lesions. Transverse sections were prepared from carious teeth in the sclerotic zone and normal hard dentin. A double-immunolabeling technique was performed on these sections, with anti-type I collagen and anti-chondroitin 4/6 sulfate monoclonal primary antibodies. We used gold-conjugated secondary antibodies to visualize the distribution of intact collagen fibrils and proteoglycans by high-resolution SEM. For sclerotic dentin, labeling densities were 19.57 ± 3.01/µm² for collagen and 9.84 ± 2.62/µm² for proteoglycans. For normal hard dentin, values were 35.20 ± 2.73/µm² and 17.03 ± 1.98/µm², respectively. Distribution of intact collagen fibrils and proteoglycans in sclerotic dentin was significantly lower than in normal hard dentin. Reductions in antigenicity from the organic matrix of sclerotic dentin under caries lesions raise concern about the potential of intrafibrillar remineralization.

KEY WORDS: caries • collagen fibrils • proteoglycans • immunohistochemistry • FEISEM

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