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Human Saliva Proteome and Transcriptome

¹ School of Dentistry and Dental Research Institute,
² UCLA Mass Spectrometry and Proteomics Center,
³ Department of Chemistry and Biochemistry,
⁴ Department of Biological Chemistry, School of Medicine,
⁵ Jonsson Comprehensive Cancer Center,
⁶ Molecular Biology Institute;
⁷ Division of Head & Neck Surgery/Otolaryngology, School of Medicine, and
⁸ Henry Samueli School of Engineering and Applied Science, University of California Los Angeles, Los Angeles, CA 90095–1668, USA

^* corresponding author, dtww{at}ucla.edu

This paper tests the hypothesis that salivary proteins and their counterpart mRNAs co-exist in human whole saliva. Global profiling of human saliva proteomes and transcriptomes by mass spectrometry (MS) and expression microarray technologies, respectively, revealed many similarities between saliva proteins and mRNAs. Of the function-known proteins identified in saliva, from 61 to 70% were also found present as mRNA transcripts. For genes not detected at both protein and mRNA levels, we made further efforts to determine if the counterpart is present. Of 19 selected genes detected only at the protein level, the mRNAs of 13 (68%) genes were found in saliva by RT-PCR. In contrast, of many mRNAs detected only by microarrays, their protein products were found in saliva, as reported previously by other investigators. The saliva transcriptome may provide preliminary insights into the boundary of the saliva proteome.

KEY WORDS: microarray • mass spectrometry • saliva proteome • saliva transcriptome • correlation analysis

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