HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Appendix Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Ishibashi, K. Articles by Abe, K. Search for Related Content PubMed PubMed Citation Articles by Ishibashi, K. Articles by Abe, K.

Roles of CLCA and CFTR in Electrolyte Re-absorption from Rat Saliva

¹ Departments of Functional Bioscience,
² Physiological Science & Molecular Biology, and
³ Morphological Biology, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan

^* corresponding author, junyama{at}college.fdcnet.ac.jp

A molecular basis for Cl^– re-absorption has not been well-characterized in salivary ductal cells. Previously, we found strong expression of a rat homologue proposed to be Ca²⁺-dependent Cl^– channels (rCLCA) in the intralobular ducts of the rat submandibular gland. To address the question as to whether rCLCA and cystic fibrosis transmembrane conductance regulator (CFTR) are involved in Cl^– re-absorption, we evaluated the electrolyte content of saliva from glands pre-treated with a small interfering RNA (siRNA). Retrograde injection into a given submandibular duct of an siRNA designed to knock down either rCLCA or CFTR reduced the expression of each of the proteins. rCLCA and CFTR siRNAs significantly increased Cl^– concentration in the final saliva during pilocarpine stimulation. These results represent the first in vivo evidence for a physiological significance of rCLCA, along with CFTR, in transepithelial Cl^– transport in the ductal system of the rat submandibular gland.

KEY WORDS: salivary • duct • re-absorption • chloride channel • siRNA

This article has been cited by other articles:

				D. Heitzmann and R. Warth Physiology and Pathophysiology of Potassium Channels in Gastrointestinal Epithelia Physiol Rev, July 1, 2008; 88(3): 1119 - 1182. [Abstract] [Full Text] [PDF]

				K. Ishibashi, K. Okamura, and J. Yamazaki Involvement of apical P2Y2 receptor-regulated CFTR activity in muscarinic stimulation of Cl- reabsorption in rat submandibular gland Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1729 - R1736. [Abstract] [Full Text] [PDF]