HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Lee, M.H. Articles by Oh, S.B. Search for Related Content PubMed PubMed Citation Articles by Lee, M.H. Articles by Oh, S.B.

Eugenol Inhibits Calcium Currents in Dental Afferent Neurons

M.H. Lee, K.-Y. Yeon, C.-K. Park, H.-Y. Li, Z. Fang, M.S. Kim, S.-Y. Choi, S.J. Lee, S. Lee², K. Park, J.-H. Lee, J.S. Kim, and S.B. Oh^1,*

¹ Department of Physiology, and
² Department of Craniomaxillofacial Cell and Developmental Biology, College of Dentistry and Dental Research Institute, Seoul National University, 28-2 Yeongeon-Dong Chongno-Ku Seoul, Seoul 110-749, Korea;

^* corresponding author, odolbae{at}snu.ac.kr

Eugenol is a topical analgesic agent widely used in the dental clinic. To elucidate the molecular mechanism underlying its analgesic action, we investigated the effect of eugenol on high-voltage-activated calcium channel (HVACC) currents in dental primary afferent neurons, and with a heterologous expression system. Dental primary afferent neurons were identified by retrograde labeling with a fluorescent dye, DiI. Eugenol inhibited HVACC currents in both capsaicin-sensitive and capsaicin-insensitive dental primary afferent neurons. The HVACC inhibition by eugenol was not blocked by capsazepine, a competitive transient receptor potential vanilloid 1 (TRPV1) antagonist. Eugenol inhibited N-type calcium currents in the cell line C2D7, stably expressing the human N-type calcium channels, where TRPV1 was not endogenously expressed. Our results suggest that the HVACC inhibition by eugenol in dental primary afferent neurons, which is not mediated by TRPV1 activation, might contribute to eugenol’s analgesic effect. Abbreviations: high-voltage-activated calcium channel, HVACC; transient receptor potential vanilloid 1, TRPV1; trigeminal ganglion, TG; dorsal root ganglion, DRG; capsazepine, CZP.

KEY WORDS: eugenol • transient receptor potential vanilloid 1 • trigeminal ganglion • C2D7 cells • high-voltage-activated calcium currents

This article has been cited by other articles:

				G. Chung, J.N. Rhee, S.J. Jung, J.S. Kim, and S.B. Oh Modulation of CaV2.3 Calcium Channel Currents by Eugenol J. Dent. Res., February 1, 2008; 87(2): 137 - 141. [Abstract] [Full Text] [PDF]

				H.Y. Li, C.-K. Park, S.J. Jung, S.-Y. Choi, S.J. Lee, K. Park, J.S. Kim, and S.B. Oh Eugenol Inhibits K+ Currents in Trigeminal Ganglion Neurons J. Dent. Res., September 1, 2007; 86(9): 898 - 902. [Abstract] [Full Text] [PDF]

				C.-K. Park, H.Y. Li, K.-Y. Yeon, S.J. Jung, S.-Y. Choi, S.J. Lee, S. Lee, K. Park, J.S. Kim, and S.B. Oh Eugenol inhibits sodium currents in dental afferent neurons. J. Dent. Res., October 1, 2006; 85(10): 900 - 904. [Abstract] [Full Text] [PDF]