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NO-cGMP Signaling Molecules in Cells of the Rat Molar Dentin-Pulp Complex

¹ Department of Operative and Preventive Dentistry and Endodontics, Heinrich-Heine-University, Moorenstrasse 5, 40225 Düsseldorf, Germany;
² Department of Operative Dentistry and Periodontology, University of Cologne, Germany;
³ Department of Anatomy I, University of Cologne;
⁴ Department of Anatomy II, University of Cologne;
⁵ Department of Pharmacology, Faculty of Medicine, 1 King’ College Circle, Toronto, ON, Canada; and
⁶ Department of Molecular and Cellular Sports Medicine, German Sports University, Cologne;

^* corresponding author, yueksel.korkmaz{at}uni-duesseldorf.de

By the formation of cyclic guanosine 3',5'-monophosphate (cGMP), nitric oxide (NO)-sensitive enzyme-soluble guanylate cyclase (sGC) plays a receptor role for NO within the NO-cGMP signaling cascade, which is involved in vasodilatation and neurotransmission. The hypothesis that NO-cGMP signaling molecules modulate cells of the dentin-pulp complex was investigated in rat molars by histochemical, immunohistochemical, immuno-ultrastructural, and organ bath techniques. NO synthase (NOS) I-III, the sGC ₂-subunit/ß₁-subunit, and cGMP were detected in odontoblasts and blood vessels. NOS I, sGC ₂, and cGMP were identified in nerve fibers. Treatment of rat molars with the NO donor NONOate (10^–5 M) increased cGMP staining intensities in blood vessels and odontoblasts, while NO synthase inhibitor L-NAME (10^–4 M) attenuated intensity of the reaction products for cGMP, suggesting an effect of endogenous NO on sGC. These correlations of patterns and alterations of cGMP staining intensities after treatment with the NO donor or NO inhibitor might represent an NO-sGC-cGMP signaling-dependent modulation of odontoblasts, blood vessels, and nerve fibers in the dentin-pulp complex.

KEY WORDS: nitric oxide • nitric oxide synthase • soluble guanylate cyclase • cyclic guanosine 3',5'-monophosphate • dentin-pulp complex

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