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RESEARCH REPORT |
1 University Federal of São Paulo, Department of Otorhinolaryngology and Human Communication Disorders, São Paolo, Brazil;
2 Department of Cytokine Biology, The Forsyth Institute, 140 The Fenway, and Department of Oral and Developmental Biology, Harvard Medical School, Boston, MA 02115, USA; and
3 Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA;
* corresponding author, pyelick{at}forsyth.org
The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.
KEY WORDS: tooth tissue engineering • dental stem cells
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