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Epitope Mapping of Porphyromonas gingivalis Heat-shock Protein and Human Heat-shock Protein in Human Atherosclerosis

¹ Department of Periodontology and Research Institute for Oral Biotechology, School of Dentistry, ² Department of Thoracic and Cardiovascular Surgery, School of Medicine, ³ Department of Molecular Biology, College of Natural Sciences, ⁴ Department of Pharmacology, and ⁵ Department of Microbiology, School of Medicine, Pusan National University, 1–10, Ami-Dong, Seo-Ku, Pusan 602–739, Korea;

^* corresponding author, jrapa{at}pusan.ac.kr

To identify T- and/or cross-reactive B-cell epitopes of P. gingivalis and human heat-shock protein (HSP)60 in atherosclerosis patients, we synthesized 104 overlapping synthetic peptides spanning whole molecules of P. gingivalis HSP60 and human HSP60, respectively. T-cell epitopes of P. gingivalis HSP were identified with the use of previously established P. gingivalis HSP-reactive T-cell lines. B-cell epitopes of P. gingivalis HSP60 and human HSP60 were identified by the use of patients’ sera. Anti-P. gingivalis, anti-P. gingivalis HSP60, or anti-human HSP60 IgG antibody titers were higher in the atherosclerosis patients compared with the healthy subjects. Five immunodominant peptides of P. gingivalis HSP60, identified as T-cell epitopes, were also found to be B-cell epitopes. Moreover, 6 cross-reactive B-cell epitopes of human HSP60 were identified. It was concluded that P. gingivalis HSP60 might be involved in the immunoregulatory process of atherosclerosis, with common T- and/or B-cell epitope specificities and with cross-reactivity with human HSP60.

KEY WORDS: Porphyromonas gingivalis • heat-shock protein • epitope • atherosclerosis

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