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RESEARCH REPORT |
1 Department of Oral Biology and
2 Restorative Dentistry, School of Dental Medicine, State University of New York at Buffalo, 310 Foster Hall, SUNY at Buffalo Main Street Campus, 3435 Main Street, Buffalo, NY 14214, USA;
* corresponding author, edgerto{at}buffalo.edu
Salivary histatin 5 (Hst 5) kills the fungal pathogen C. albicans via a mechanism that involves binding and subsequent efflux of cellular ATP. Our aims were to identify inorganic ions found in saliva that influence Hst 5 fungicidal activity. Increasing ionic strength with relevant salivary anions (Cl- and CO3-) did not reduce Hst 5 binding or uptake by yeast cells, but reduced the Hst-induced efflux of ATP. Extracellular MgCl2 (25 mM) maximally inhibited 30–40% of Hst 5 killing with 40% reduction in ATP efflux, while pre-treatment of cells with only 2 mM CaCl2 inhibited 80–90% of killing, and prevented ATP efflux. Loss of fungicidal activity by the addition of CaCl2 or MgCl2 was a result of inhibition of binding of Hst 5 to C. albicans cells. Calcium is a potent inhibitor of Hst 5 candidacidal activity at physiological concentrations and may be the primary salivary ion responsible for the masking effect of saliva.
KEY WORDS: Candida albicans • histatin • saliva • calcium
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