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Bone with a Vascular Flap Induced from Fat Tissue with the Use of rhBMP-2 in Rats

¹ Oral Surgery, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo, Tokyo 113-8549, Japan;
² Department of Dentistry and Oral Surgery, Hirosaki University School of Medicine, 5, Zaifu-cho, Hirosaki, Aomori 036-8216, Japan; and
³ Instrumental Analysis Research Center, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo, Tokyo 113-8519, Japan;

^* corresponding author, maruoka.osur{at}tmd.ac.jp

Here we report that successful bone formation with a vascular flap inside a cylindrical mold was induced from fat tissue with the use of recombinant human bone morphogenetic protein-2 in rats. Fat tissue connected to blood vessels was prepared to fit into the mold and implanted intramuscularly into the hind leg in Wistar rats. RhBMP-2 (20 µg) was applied in a collagen sheet previously placed on the inside surface of the mold. Bone formation was confirmed radiologically and morphologically at 2, 4, and 8 weeks after the surgery. In the control group without rhBMP-2 or the group with ligation of the blood vessels before the implantation, bone formation was not observed. Our success in bone formation having a definite size, shape, and blood supply may lead to a therapeutic approach to effective bone reconstitution. The present study is the first report on bone induction from fat tissue by rhBMP-2 in vivo.

KEY WORDS: recombinant human bone morphogenetic protein-2 (rhBMP-2) • bone formation within a mold • vascularized bone flap • bone induction from fat tissue