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J Dent Res 82(7): 542-545, 2003
© 2003 International and American Associations for Dental Research

RESEARCH REPORT
Biological

Expression and Localization of TREK-1 K+ Channels in Human Odontoblasts

H. Magloire1,*, F. Lesage2, M.L. Couble1, M. Lazdunski2, and F. Bleicher1

1 Laboratoire du Développement des Tissus Dentaires, EA 1892, IFR 62, Faculté d’Odontologie, Rue G. Paradin, 69372, Lyon cedex 08, France; and
2 Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UPR 441, Sophia Antipolis, 06560 Valbonne, France;

*corresponding author, magloire{at}laennec.univ-lyon1.fr

During tooth development, odontoblasts are the cells that form dentin and possibly mediate early stages of sensory processing in teeth. It is suggested that ion channels assist in these events. Indeed, mechanosensitive potassium currents, transducing mechanical stimuli into electrical cell signals, have been previously recorded in the human odontoblast cell membrane. Here, we show by RT-PCR that the mechanosensitive potassium channel TREK-1 (a member of the two-pore-domain potassium channel family) is overexpressed in these cultured cells compared with pulp cells in vitro. In situ hybridization showed that transcripts are detected in the odontoblast layer in vivo. The use of antibodies shows that TREK-1 is strongly expressed in the membrane of coronal odontoblasts and absent in the root. This distribution is related to the spatial distribution of nerve endings identified by labeling of the low-affinity nerve growth factor (NGF) receptor (p75NTR). These results demonstrate the expression of TREK-1 in human odontoblasts in vitro and in vivo.

KEY WORDS: K+ channels • odontoblast • teeth




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