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1 Department of Oral Biology, School of Dentistry, University of Missouri-Kansas City, 650 E. 25th Street, Kansas City, MO 64108, USA;
2 Department of Orthopaedic Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China;
3 Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences/NIH Research Triangle Park, NC;
4 Graduate School of Natural Science and Technology, Okayama University;
#corresponding author, fengj{at}umkc.edu
Dentin Matrix Protein 1 (Dmp1) was originally identified from dentin. However, its expression and function in vivo are not clear. To clarify these two issues, we have generated mice carrying a truncated Dmp1 gene by using gene targeting to replace exon 6 with a lacZ gene. Northern blot analysis shows the expected 5.8-kb Dmp1-lacZ fusion transcript and loss of the wild-type 2.8-kb Dmp1 transcript, confirmed by a lack of immunostaining for the protein. Using heterozygous animals, we demonstrate that Dmp1 is specific for mineralized tissues. Not previously shown, Dmp1 is also expressed in pulp cells. Dmp1-deficient embryos and newborns display no apparent gross abnormal phenotype, although there are a modest expansion of the hypertrophic chondrocyte zone and a modest increase in the long bone diameter. This suggests that DMP1 is not essential for early mouse skeletal or dental development.
KEY WORDS: Dmp1 expression • bone • tooth • development • knockout mice
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