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¹H and ¹³C NMR Spectroscopic Analysis of Human Saliva

¹ Medical Unit, St. Bartholomews and the Royal London School of Medicine and Dentistry, London E1 1BB, UK; and
² School of Dentistry, The Queen's University of Belfast, Royal Victoria Hospital, Grosvenor Road, Belfast BT12 6BP, Northern Ireland;

*corresponding author, Room AW518, Medical Unit, 5th floor, Alexandra Wing, Royal London Hospital, London E1 1BB, UK, m.grootveld{at}qmul.ac.uk

We have explored the ability of high-resolution NMR techniques to (1) index salivary biomolecules and (2) provide valuable data regarding intra- and inter-subject variability in the concentrations of a series of components readily determinable by this technique (organic acids and malodorous amines). Experiments were conducted on ‘whole’ saliva samples collected from 20 patients, either randomly during their daily activities, or, for investigations involving the quantification of salivary biomolecules, immediately after they woke in the morning throughout a three-day period. These NMR techniques permitted us to detect greater than 60 metabolites, together with agents arising from dietary, oral health care product, and pharmaceutical sources. Highly significant "between-subject" differences in the a.m. waking salivary metabolite concentrations were found for 9 out of 11 components monitored. It is concluded that NMR spectroscopy serves as a powerful technique for the multicomponent analysis of human saliva.

KEY WORDS: human saliva • multicomponent analysis • NMR spectroscopy • periodontal diseases • metabolic profile

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