HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Houri-Haddad, Y. Articles by Shapira, L. Search for Related Content PubMed PubMed Citation Articles by Houri-Haddad, Y. Articles by Shapira, L.

Interferon-gamma Deficiency Attenuates Local P. gingivalis-induced Inflammation

¹ Department of Periodontology and
² Department of Oral Biology, Faculty of Dental Medicine, Hadassah and Hebrew University Medical Centers, PO Box 12272, Jerusalem 91120, Israel;

*corresponding author, shapiral{at}cc.huji.ac.il

Infection with the periodontal pathogen Porphyromonas gingivalis causes a strong local inflammatory reaction. Using IFN-deficient mice, we tested the hypothesis that the absence of IFN would result in a reduction of the local pro-inflammatory response to P. gingivalis. Cytokine secretion by macrophages from IFN^-/- animals was significantly attenuated. Addition of IFN restored cytokine secretion. In vivo injection of P. gingivalis into subcutaneous chambers increased the intra-chamber leukocyte counts and TNF and IL-1ß levels. This increase was significantly lower in the IFN^-/- mice. Local reconstitution of IFN^-/- mice at the site of inflammation with the IFN gene increased the levels of TNF and decreased the IL-10 levels. Anti-P. gingivalis IgG1 levels, a marker of Th2 response, were higher in immunized IFN^-/- than in IFN^+/+ mice. The results suggest that lack of IFN reduced the amplitude of the local pro-inflammatory response without decreasing the humoral protective response. The higher IgG1/IgG2a ratio observed supports the possibility of a Th2-dominant response in IFN-deficient animals.

KEY WORDS: IFN deficiency • Porphyromonas gingivalis • inflammation • cytokines • antibody

This article has been cited by other articles:

				P. Stashenko, R. B. Goncalves, B. Lipkin, A. Ficarelli, H. Sasaki, and A. Campos-Neto Th1 Immune Response Promotes Severe Bone Resorption Caused by Porphyromonas gingivalis Am. J. Pathol., January 1, 2007; 170(1): 203 - 213. [Abstract] [Full Text] [PDF]