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J Dent Res 81(6): 392-394, 2002
© 2002 International and American Associations for Dental Research

RESEARCH REPORT
Biological

The Expression of Dentin Sialophosphoprotein Gene in Bone

C. Qin1,*, J.C. Brunn1, E. Cadena1, A. Ridall1, H. Tsujigiwa2, H. Nagatsuka2, N. Nagai2, and W.T. Butler1

1 Department of Basic Sciences, The University of Texas-Houston Health Science Center Dental Branch, 6516 M.D. Anderson Boulevard, DBB Rm 4.133, Houston, TX 77030, USA; and
2 Department of Oral Pathology, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700, Japan;

*corresponding author, cqin{at}mail.db.uth.tmc.edu

Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) are expressed as a single mRNA transcript coding for a large precursor protein termed dentin sialophosphoprotein (DSPP). DSP, DPP, and DSPP have been considered to be tooth-specific. To test for the expression of the dspp gene in bone, we performed Western immunoblots and reverse-transcription polymerase chain-reaction (RT-PCR). With Western immunoblots, we detected DSP in the Gdm/EDTA extracts of rat long bone, at a level of about 1/400 of that in dentin. Using RT-PCR, we detected DSPP mRNA in mouse calvaria. Similar to Western immunoblots, the results of RT-PCR indicated that the dspp gene is expressed at a lower level in bone than in dentin and odontoblasts. Analysis of the data shows that DSPP is not a tooth-specific protein, and that dramatically different regulatory mechanisms governing DSPP expression are involved in the bone and dentin.

KEY WORDS: dentin sialophosphoprotein • dentin sialoprotein • dentin phosphoprotein • gene expression • bone




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