JDR JDR Most Cited Articles
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iwata, T.
Right arrow Articles by Oida, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iwata, T.
Right arrow Articles by Oida, S.
J Dent Res 81(6): 387-391, 2002
© 2002 International and American Associations for Dental Research

RESEARCH REPORT
Biological

Noggin Blocks Osteoinductive Activity of Porcine Enamel Extracts

T. Iwata1,*, Y. Morotome1, T. Tanabe2, M. Fukae2, I. Ishikawa1, and S. Oida2

1 Section of Periodontology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; and
2 Department of Biochemistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Kanagawa 230-8501, Japan;

*corresponding author, takanori.peri{at}tmd.ac.jp

Enamel extracts induce biomineralization such as osteogenesis and cementogenesis, but the molecular component responsible for this activity remains uncertain. We fractionated enamel extracts from developing pig teeth and isolated the osteoinductive fraction. Proteins from pig enamel scrapings were extracted under alkaline conditions (pH 10.8) and fractionated with the use of a Sephadex G-100 (size exclusion) column. The ability of each fraction to enhance alkaline phosphatase (ALP) activity was assayed in ST2 cells, a mouse bone marrow stromal cell line. The osteoinductive fraction of enamel extracts (OFE) was found in fractions 44 and 45, which induced ST2 cells to express the phenotype of bone-forming osteoblasts, and to form mineralized nodules. Furthermore, the ALP activity of ST2 cells exposed to OFE was reduced by noggin, an antagonist of BMPs, and OFE reacted with BMP-2/4 antibody in dot-blot analysis. These results indicate that OFE contains BMPs that contribute to the induction of biomineralization.

KEY WORDS: enamel extracts • bone morphogenetic proteins (BMPs) • noggin.




This article has been cited by other articles:


Home page
 J. Dent. Res.Home page
S. Suzuki, T. Nagano, Y. Yamakoshi, K. Gomi, T. Arai, M. Fukae, T. Katagiri, and S. Oida
Enamel Matrix Derivative Gel Stimulates Signal Transduction of BMP and TGF-{beta}
J. Dent. Res., June 1, 2005; 84(6): 510 - 514.
[Abstract] [Full Text] [PDF]

Home page
 Crit. Rev. Oral Biol. Med.Home page
E. Venezia, M. Goldstein, B.D. Boyan, and Z. Schwartz
THE USE OF ENAMEL MATRIX DERIVATIVE IN THE TREATMENT OF PERIODONTAL DEFECTS: A LITERATURE REVIEW AND META-ANALYSIS
Crit. Rev. Oral. Biol. Med., November 1, 2004; 15(6): 382 - 402.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
IADR Journals Advances in Dental Research ®
Journal of Dental Research ® Critical Reviews (1990-2004)
Copyright © 2002 Institutional Access Guidelines