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J Dent Res 81(11): 761-766, 2002
© 2002 International and American Associations for Dental Research

RESEARCH REPORT
Clinical

Molecular and Cultural Analysis of the Microflora Associated with Endodontic Infections

M.A. Munson1, T. Pitt-Ford2, B. Chong2, A. Weightman3, and W.G. Wade1,*

1 Department of Microbiology and
2 Department of Conservative Dentistry, Guy’s, King’s, and St. Thomas’ Dental Institute, Guy’s Tower, Guy’s Hospital, King’s College London, London SE1 9RT, UK; and
3 Cardiff School of Biosciences, Cardiff University, Cardiff, UK;

* corresponding author, william.wade{at}kcl.ac.uk

Cultural studies have indicated that a subset of the oral microflora is responsible for endodontic infections. Approximately 50% of oral bacteria are unculturable, so it is likely that currently unknown bacteria are present in such infections. In this study, cultural and molecular analyses were performed on the microflora in aspirate samples collected from 5 infected root canals. 16S rDNA sequences from 261 isolates and 624 clones were identified by comparison with database sequences. Sixty-five taxa were identified, of which 26 were found by the molecular method alone. A mean of 20.2 taxa was found in each sample. A new species of Dialister was the only organism present in all 5 samples. Twenty-seven novel taxa were detected, 18 of which belonged to the phylum Firmicutes and 8 to Bacteroidetes. Culture-independent, molecular analysis has revealed a more diverse microflora associated with endodontic infections than that revealed by cultural methods alone.

KEY WORDS: endodontic • microbiology • 16S rRNA • unculturable • molecular




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