Journal of Dental Research, Vol 80, 1772-1776, Copyright © 2001 by International & American Associations for Dental Research Online Journals
Preparation of and tissue response to DNA-lipid films
T. Fukushima, Y. Inoue, T. Hayakawa, K. Taniguchi, K. Miyazaki and Y. Okahata
Department of Dental Materials, Fukuoka Dental College, Japan. tadaof@college.fdcnet.ac.jp
DNA-containing films have the potential to form complexes with antibiotics
or cytokines by intercalation or groove-binding. This principle can be
highly relevant for regenerative wound-healing around oral implants and in
periodontology. In this study, we prepared DNA-lipid films and examined
tissue responses to them as an indicator of their biological properties.
The lipids were synthesized from the reaction of L-alanine, n-alkyl
alcohol, and p-toluenesulfonic acid. We prepared the self-standing,
water-insoluble DNA-lipid films by casting the DNA-lipid complex from
chloroform/ethanol solution. The DNA-lipid complexes, which had 1:1 ratios
of phosphate anions to cationic lipid, were found to have a double-helical
B-form structure. The DNA-lipid films were almost dissolved 3 days after
subcutaneous implantation in the backs of rats. There were no inflammatory
reactions or inhibition of new tissue formation. We concluded that
DNA-lipid films can be prepared by simple methods, and that they do not
cause an unfavorable tissue response.
