Journal of Dental Research, Vol 77, 1497-1503, Copyright © 1998 by International & American Associations for Dental Research Online Journals

ARTICLES

HLA-DR alleles are associated with IDDM, but not with impaired neutrophil chemotaxis in IDDM

C. J. Gustke, S. H. Stein, T. C. Hart, W. H. Hoffman, P. J. Hanes, C. M. Russell, G. S. Schuster and S. C. Watson
US Public Health Service, Indian Health Service, Tohatchi Health Center, NM, USA.

Insulin-dependent diabetes mellitus (IDDM) is a risk factor for periodontitis. Depressed neutrophil chemotaxis has been demonstrated in IDDM and in early-onset periodontitis (EOP). HLA-DR antigens are associated with both IDDM and periodontitis. This investigation sought to determine an association of HLA-DR3, -DR4, and -DR53 with impaired neutrophil chemotaxis in an IDDM sample. The neutrophil chemotaxis index of 41 diabetics and 27 controls was determined by a modified Boyden chamber method, and certain class II HLA genotypes were determined by polymerase chain-reaction amplification of genomic DNA by means of sequence-specific primers (PCR-SSP). The mean chemotaxis index of the diabetics was significantly less than that of the controls (p < or = 0.02). HLA-DR3 (p < or = 0.002), -DR4 (p < 0.003), and -DR53 (p < or = 0.001) were associated with IDDM. Neutrophil chemotaxis and glucose metabolism were not significantly correlated. None of the HLA-DR alleles was associated with impaired neutrophil chemotaxis. Therefore, the neutrophil chemotaxis defect of IDDM appears to be independent of these HLA-DR-associated genes.

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