Journal of Dental Research, Vol 77, 545-554, Copyright © 1998 by International & American Associations for Dental Research Online Journals

ARTICLES

Bone morphogenetic protein-7 (osteogenic protein-1, OP-1) and tooth development

M. N. Helder, H. Karg, T. J. Bervoets, S. Vukicevic, E. H. Burger, R. N. D'Souza, J. H. Woltgens, G. Karsenty and A. L. Bronckers
Department of Oral Cell Biology, ACTA, Vrije Universiteit, Amsterdam, The Netherlands.

Bone morphogenetic proteins (BMPs) form a family of growth factors originally isolated from extracellular bone matrix that are capable of inducing bone formation ectopically. We studied the expression, tissue localization, and function of BMP-7 (OP-1) during tooth development in rodents. Patterns of BMP-7 gene expression and peptide distribution indicated that BMP-7 was present in dental epithelium during the dental lamina, bud, and cap stages. During the bell stage, BMP-7 mRNA expression and protein distribution shifted from dental epithelium toward the dental mesenchyme. With advancing differentiation of odontoblasts, BMP-7 protein staining in the dental papilla became restricted to the layer of fully functional odontoblasts in the process of depositing (pre)dentin. Secretory-stage ameloblasts exhibited weak immunostaining for BMP-7. A restricted pattern of staining in ameloblasts became apparent in post-secretory stages of amelogenesis. Also, cells of the forming periodontal ligament were immunopositive. Histological analysis of tooth development in neonatal BMP-7-deficient mice did not reveal obvious changes compared with wild-type mice. We conclude that, in developing dental tissues, BMP-7 has distribution and expression patterns similar to those of other BMP members but is not an essential growth factor for tooth development, possibly because of functional redundancy with other BMP members or related growth factors.

This article has been cited by other articles:

				O. M. Onagbesan, V. Bruggeman, P. Van As, K. Tona, J. Williams, and E. Decuypere BMPs and BMPRs in chicken ovary and effects of BMP-4 and -7 on granulosa cell proliferation and progesterone production in vitro Am J Physiol Endocrinol Metab, November 1, 2003; 285(5): E973 - E983. [Abstract] [Full Text] [PDF]

				T. Thyagarajan, S. Totey, M. J. S. Danton, and A. B. Kulkarni GENETICALLY ALTERED MOUSE MODELS: THE GOOD, THE BAD, ANDTHE UGLY Crit. Rev. Oral. Biol. Med., May 1, 2003; 14(3): 154 - 174. [Abstract] [Full Text] [PDF]

				K. Sena, Y. Morotome, O. Baba, T. Terashima, Y. Takano, and I. Ishikawa Gene Expression of Growth Differentiation Factors in the Developing Periodontium of Rat Molars J. Dent. Res., March 1, 2003; 82(3): 166 - 171. [Abstract] [Full Text] [PDF]

				M. Zhao, J.E. Berry, and M.J. Somerman Bone Morphogenetic Protein-2 Inhibits Differentiation and Mineralization of Cementoblasts in vitro J. Dent. Res., January 1, 2003; 82(1): 23 - 27. [Abstract] [Full Text] [PDF]

				C. Muehleman, K. E. Kuettner, D. C. Rueger, P. ten Dijke, and S. Chubinskaya Immunohistochemical Localization of Osteogenetic Protein (OP-1) and Its Receptors in Rabbit Articular Cartilage J. Histochem. Cytochem., October 1, 2002; 50(10): 1341 - 1350. [Abstract] [Full Text] [PDF]

				T. Iwata, Y. Morotome, T. Tanabe, M. Fukae, I. Ishikawa, and S. Oida Noggin Blocks Osteoinductive Activity of Porcine Enamel Extracts J. Dent. Res., June 1, 2002; 81(6): 387 - 391. [Abstract] [Full Text] [PDF]

				H. C. Anderson, P. T. Hodges, X. M. Aguilera, L. Missana, and P. E. Moylan Bone Morphogenetic Protein (BMP) Localization in Developing Human and Rat Growth Plate, Metaphysis, Epiphysis, and Articular Cartilage J. Histochem. Cytochem., November 1, 2000; 48(11): 1493 - 1502. [Abstract] [Full Text]

				D. G. Monroe, D. F. Jin, and M. M. Sanders Estrogen Opposes the Apoptotic Effects of Bone Morphogenetic Protein 7 on Tissue Remodeling Mol. Cell. Biol., July 1, 2000; 20(13): 4626 - 4634. [Abstract] [Full Text]

				S. Chubinskaya, C. Merrihew, G. Cs-Szabo, J. Mollenhauer, J. McCartney, D. C. Rueger, and K. E. Kuettner Human Articular Chondrocytes Express Osteogenic Protein-1 J. Histochem. Cytochem., February 1, 2000; 48(2): 239 - 250. [Abstract] [Full Text]

				A. Veis, K. Tompkins, K. Alvares, K. Wei, L. Wang, X. S. Wang, A. G. Brownell, S.-M. Jengh, and K. E. Healy Specific Amelogenin Gene Splice Products Have Signaling Effects on Cells in Culture and in Implants in Vivo J. Biol. Chem., December 22, 2000; 275(52): 41263 - 41272. [Abstract] [Full Text] [PDF]