Journal of Dental Research, Vol 77, 1965-1969, Copyright © 1998 by International & American Associations for Dental Research Online Journals
Continuous administration of basic fibroblast growth factor (FGF-2) accelerates bone induction on rat calvaria--an application of a new drug delivery system
T. Kimoto, R. Hosokawa, T. Kubo, M. Maeda, A. Sano and Y. Akagawa
Department of Removable Prosthodontics, Hiroshima University School of Dentistry, Japan.
Some studies have shown that locally applied basic fibroblast growth factor
(FGF-2) enhances bone regeneration at a fracture site, while others have
not been in agreement. We developed a new continuous FGF-2 delivery system
designed to accelerate cytokine-induced new bone formation. A subperiosteal
pocket was surgically formed in 36 eight-week-old male Wistar rats. The
rats were administered 0, 1, 10, or 100 ng of FGF-2 contained in a collagen
minipellet, mixed with allogeneic demineralized bone matrix in a
dome-shaped Millipore filter and then placed into the pocket. New bone
formation in the dome was evaluated at 2, 4, and 8 wks after placement.
Soft x-ray radiographs disclosed an apparently larger radiopaque region in
the 1-ng group at 4 wks compared with those in the other groups.
Morphometrical analysis revealed that the new bone area in the 1-g group
was significantly larger than that in the 0-g group (p<0.01). In the
100-ng FGF-2 group, new bone formation seemed suppressed. We concluded that
continuous slow administration of a small amount of FGF-2 accelerates
bone-derived osteogenic cytokine-induced new bone formation.
