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Journal of Dental Research, Vol 76, 1244-1249, Copyright © 1997 by International & American Associations for Dental Research Online Journals

ARTICLES

Localization and expression of CSF-1 receptor in rat dental follicle cells

G. E. Wise, L. Zhao and R. L. Grier 4th
Department of Veterinary Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803-8408, USA.

Colony-stimulating factor-1 (CSF-1) accelerates tooth eruption in rats and is localized in the dental follicle, a loose connective tissue sac that is necessary for eruption to occur. CSF-1 enhances the cellular events that occur in the follicle prior to eruption--namely, an influx of monocytes into the follicle early post-natally to form the osteoclasts needed to resorb bone for the eruption pathway. Because CSF-1 levels are at a peak at day 3 post-natally, and because CSF-1 has an autocrine effect on its own gene expression, the question remains as to what causes the subsequent decline in CSF-1 protein and mRNA after day 3 post-natally. To determine if the autocrine effect is inhibited through the CSF-1 receptor, analysis of the CSF-1 receptor mRNA levels in cultured dental follicle cells reveals that high concentrations of CSF-1 reduce the gene expression of the CSF-1 receptor. Interleukin 1 alpha, a molecule that enhances CSF-1 gene expression, has no effect on CSF-1 receptor mRNA levels. Immunostaining for the CSF-1 receptor protein shows that it is present in the dental follicle early post-natally and is either absent or greatly reduced by day 10 post-natally. Earlier studies showed that the mRNA levels of the CSF-1 receptor also parallel this time course. Thus, the above results suggest that the feedback inhibition of the autocrine effect of CSF-1 on its own expression is through the effect of CSF-1 inhibiting the translation and transcription of its receptor. In turn, these molecular interactions possibly regulate the cellular events that occur in the follicle prior to and during eruption.


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 Crit. Rev. Oral Biol. Med.Home page
G.E. Wise, S. Frazier-Bowers, and R.N. D'Souza
CELLULAR, MOLECULAR, AND GENETIC DETERMINANTS OF TOOTH ERUPTION
Crit. Rev. Oral. Biol. Med., July 1, 2002; 13(4): 323 - 335.
[Abstract] [Full Text]


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