Journal of Dental Research, Vol 74, 1072-1078, Copyright © 1995 by International & American Associations for Dental Research Online Journals
Enhanced cytokine production and collagen synthesis of gingival fibroblasts from patients with denture fibromatosis
K. Nakao, K. Yoneda and T. Osaki
Department of Oral Surgery, Kochi Medical School, Japan.
The mechanisms of denture-induced gingival hypertrophy remain to be
explored. Since fibroblast proliferation and bone resorption characterize
this disorder, the possible involvement of cytokines was investigated.
Gingival fibroblasts were obtained from six patients with denture
fibromatosis (Den-Fb) and six healthy persons (Nor-Fb). Cells were compared
for proliferation, collagen synthesis, and cytokine production.
Incorporation of [3H]thymidine (TdR) was increased in 3 Den-Fb and 3 Nor-Fb
lines in the presence of interleukin-1-beta (IL-1 beta) (10 U/mL) and tumor
necrosis factor-alpha (TNF-alpha) (from 10 to 100 U/mL). Proline
incorporation in Den-Fb was higher than that in Nor-Fb, and the mean
collagen synthesis level in Den-Fb was significantly higher than that in
Nor-Fb. Although there was no difference between the up-regulation of
protein synthesis in Den-Fb and Nor-Fb induced by IL-1 beta or TNF-alpha,
the receptors for these cytokines were expressed at higher levels in cell
lines which exhibited higher protein synthesis. Between Nor-Fb and Den-Fb,
there was no difference in the generation of granulocyte-macrophage
colony-stimulating factor (GM-CSF) or interleukin-6 (IL-6). However, most
Den-Fb produced more GM-CSF and IL-6 in the presence of TNF-alpha.
Enhancement of IL-6 generation by GM-CSF was also more prominent in Den-Fb.
GM-CSF and IL-6 were synergistically generated after co-culture of the
fibroblasts with gingival keratinocytes. GM-CSF and IL-6 generation of
Den-Fb was markedly enhanced by co-culture of Den-Fb with peripheral blood
mononuclear cells (PBMC), especially PBMC from patients.(ABSTRACT TRUNCATED
AT 250 WORDS)
