JDR Woodhead Publishing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, Y. H.
Right arrow Articles by Bowden, G. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Y. H.
Right arrow Articles by Bowden, G. H.

Journal of Dental Research, Vol 73, 1615-1626, Copyright © 1994 by International & American Associations for Dental Research Online Journals

ARTICLES

The effect of environmental pH and fluoride from the substratum on the development of biofilms of selected oral bacteria

Y. H. Li and G. H. Bowden
Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Canada.

The present study was initiated to answer the question, "Does fluoride from the substratum influence the accumulation of bacterial cells in an associated biofilm?" 'Fluoride-bound hydroxyapatite' (FHA) and 'fluoride-free hydroxyapatite' (HA) rods were prepared as test and control surfaces, respectively. Biofilms of S. mutans BM71, A. naeslundii genospecies 2 WVU627, and L. casei BM225 accumulated on the surfaces of rods in a semi-defined, mucin-based medium in a chemostat. Culture conditions were varied from pH 4.5 to 7.0 under carbon (glucose) limitation and excess, at a dilution rate of D = 0.1 h-1. Low environmental pH reduced both the numbers of cells on HA surfaces during the early phases (from 0 to 2 h) of accumulation and the final numbers of cells in mature biofilms (20 h). The initial adherence of cells was unaffected by surface fluoride under any of the conditions tested. Similarly, biofilm cells under carbon limitation and those under carbon excess at pH 7.0 were not affected by surface fluoride. However, at low environmental pH values, pH 5.5 for S. mutans and pH 6.0 for A. naeslundii under glucose excess, the accumulation of biofilm cells on the FHA surfaces was significantly reduced (p < 0.05-0.001). Biofilm cell number doubling times of S. mutans and A. naeslundii were increased on FHA relative to HA. Biofilms of L. casei were not significantly affected, even at pH 4.5 in glucose excess. The results confirmed that fluoride from the substratum affected fluoride-sensitive biofilm cells but only under conditions of glucose excess ad low pH.


This article has been cited by other articles:


Home page
 MicrobiologyHome page
K. McNeill and I. R. Hamilton
Effect of acid stress on the physiology of biofilm cells of Streptococcus mutans
Microbiology, March 1, 2004; 150(3): 735 - 742.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
IADR Journals Advances in Dental Research ®
Journal of Dental Research ® Critical Reviews (1990-2004)
Copyright © 1994 Institutional Access Guidelines