Journal of Dental Research, Vol 70, 102-106, Copyright © 1991 by International & American Associations for Dental Research Online Journals
Analysis of neutrophil chemotaxis and CD11b expression in pre-pubertal periodontitis
K. Watanabe, L. A. Lambert, L. G. Niederman, I. Punwani and B. R. Andersen
Department of Periodontics, College of Dentistry, University of Illinois, Chicago 60612.
The objectives of this study were to (1) determine chemotaxis (CX) response
by neutrophils (PMNs) isolated from patients with the localized form of
pre-pubertal periodontitis (L-PP) and compare these responses with those of
age-matched and adult controls, (2) determine the level and up-regulation
of CD11b expression upon stimulation with fMLP by peripheral blood PMNs
(PB-PMNs) isolated from patients with L-PP and compare these levels with
those of age-matched and adult controls, and (3) determine whether there is
a correlation between CX and CD11b expression (up-regulation) by PB-PMNs.
PB-PMNs from a total of seven patients with L-PP, seven age-matched
pediatric controls, and 11 adult controls (four adults for both CD11b and
CX assays, and seven adults for CX assays only) were analyzed for CX and
CD11b expression. Direct immunofluorescence staining of CD11b was performed
with FITC-conjugated monoclonal antibody. Flow cytometry was used to
analyze stained cells for their fluorescence intensity. Chemotaxis activity
in response to 10(-8) mol/L fMLP was examined in micro-well chemotaxis
chambers. The results indicated that CX of PMNs from both L-PP patients and
pediatric control subjects was significantly decreased, compared with that
of normal adult control subjects (p less than 0.001). There was no
significant difference between CX of PMNs from L-PP patients and that from
normal pediatric controls (p greater than 0.7). CD11b expression did not
significantly differ among L-PP patients, normal pediatric controls, and
adult controls.(ABSTRACT TRUNCATED AT 250 WORDS)
