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Journal of Dental Research, Vol 69, 1184-1187, Copyright © 1990 by International & American Associations for Dental Research Online Journals

ARTICLES

Optical quantification of caries-like lesions in vitro by use of a fluorescent dye

J. W. Van de Rijke and J. J. Ten Bosch
Laboratory for Materia Technica, University of Groningen, The Netherlands.

An experimental method was developed for measurement of the fluorescence intensity of a dye that was introduced into caries-like lesions in vitro. A distinct pattern of change of fluorescence intensity with time appeared, displaying a plateau value and a peak value for each measurement. Both plateau and peak values showed a linear correlation with calcium loss, as measured with longitudinal microradiography. The correlation coefficients were r = 0.87 for plateau values and r = 0.89 for peak values. The difference in scattering by dry and wet caries lesions was also measured with the same equipment, which showed a linear correlation with calcium loss of r = -0.53.




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