Journal of Dental Research, Vol 67, 462-466, Copyright © 1988 by International & American Associations for Dental Research Online Journals
Effect of NaF on cAMP accumulation, cAMP-dependent protein kinase activity in, and amylase secretion from, rat parotid gland cells
A. R. Shahed and D. W. Allmann
Department of Biochemistry, Indiana University School of Medicine, Indianapolis 46223, USA.
Stimulation of amylase secretion from parotid glands by beta-adrenergic
agonists is mediated by the activation of adenylate cyclase and the
resultant increase in cellular cAMP. Since NaF is known to increase
adenylate cyclase activity and cAMP accumulation in intact cells, we
investigated whether it would stimulate amylase secretion from isolated rat
parotid gland cells. The results provide evidence that the addition of NaF
(0.01-10 mmol/L) increased cAMP concentration (1.5-2.8-fold) in, and
amylase secretion (16-93%) from, isolated parotid gland acinar cells. NaF
was found to increase cAMP-dependent protein kinase activity ratios
(51-84%) in a concentration- and time-dependent manner. The data suggest
that the stimulation of amylase secretion from parotid gland cells by NaF
may be mediated by an increase in the cellular cAMP concentration, which
exerts its effect, at least in part, by increasing the activity of
cAMP-dependent protein kinase.
