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Journal of Dental Research, Vol 66, 1195-1198, Copyright © 1987 by International & American Associations for Dental Research Online Journals

ARTICLES

Changes of serum alkaline phosphatase following mandibular osteotomy in the rat

L. P. Nilsson and G. Granstrom

Activity of alkaline phosphatase in serum (S-ALP) was characterized and compared with that of non-specific alkaline phosphatase (APase) of hard-tissue origin in the rat. The enzyme was characterized biochemically, and optimal incubation procedures were determined. S-ALP levels were determined before and after mandibular osteotomy combined with different degrees of periosteal reflections. Separation of S-ALP isoenzymes by isoelectric focusing revealed four major bands. A marked decrease of S-ALP activity was seen after osteotomy, and all isoenzymes were affected similarly. After treatment with hyperbaric oxygen, a smaller decrease in S-ALP was seen. S-ALP could be used as a marker for hard-tissue turn-over after surgical procedures.




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