Journal of Dental Research, Vol 66, 795-798, Copyright © 1987 by International & American Associations for Dental Research Online Journals

ARTICLES

In vitro caries-like lesion production by Streptococcus mutans and Actinomyces viscosus using sucrose and starch

B. H. Clarkson, D. Krell, J. S. Wefel, J. Crall and F. F. Feagin

We investigated the formation of caries-like lesions on root and enamel sections by S. mutans and A. viscosus when grown in four different carbohydrate substrates. The substrates were: sucrose, starch + alpha-amylase, and a combination of starch and sucrose with and without alpha-amylase. Twenty-four sections with exposed windows on both the root and enamel surfaces were exposed to the individual bacterial species in each of the four substrates for three weeks. At three weeks, the sections were removed and lesion depths measured. When grown in sucrose alone, S. mutans produced significantly deeper lesions in both root and enamel surfaces when compared with A. viscosus. However, S. mutans failed to produce lesions when grown in "limited" sucrose with starch added, whereas A. viscosus in this same substrate produced lesions in the enamel and root surfaces. A viscosus also produced either similar or significantly deeper lesions in both root and enamel surfaces, when compared with S. mutans grown in the two substrates with amylase. Thus, under these in vitro conditions, it was demonstrated that A. viscosus could utilize starch, whereas utilization of starch by S. mutans was alpha-amylase-dependent.

This article has been cited by other articles:

				C. L. Simpson and R. R. B. Russell Intracellular alpha -Amylase of Streptococcus mutans J. Bacteriol., September 1, 1998; 180(17): 4711 - 4717. [Abstract] [Full Text]