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Journal of Dental Research, Vol 66, 569-575, Copyright © 1987 by International & American Associations for Dental Research Online Journals

ARTICLES

Regulation of amino acid influx and efflux at the basolateral plasma membrane of the salivary gland epithelium: effects of parasympathetic nerve stimulation

G. E. Mann and D. L. Yudilevich

Basolateral amino acid transport systems in the salivary epithelium of resting and secreting cat submandibular glands were characterized by means of a rapid paired-tracer dilution technique. Amino acid uptake was measured by comparison of venous tracer concentration profiles for a labeled amino acid and D-mannitol (an extracellular tracer of similar size) following an intra-arterial bolus injection of both radioactive molecules. Unidirectional uptake of 21 amino acids, dopamine, noradrenaline, and serotonin was quantified in non-secreting glands. During 8 Hz parasympathetic nerve stimulation, significant epithelial uptakes were measured for L-[3H] alanine and L-[3H] phenylalanine, but less than 0.2% of the injected amino acid was recovered in the collected saliva. In non-secreting glands, cross-inhibition studies of L-[3H] alanine, L-[3H] phenylalanine, and L-[3H] lysine uptake by unlabeled amino acid competitors and detailed kinetic influx experiments indicated that epithelial uptake was mediated by three distinct parallel transport systems: ASC (short-chain neutral), L (branched-chain and aromatic neutral), and y+ (cationic). Rapid metabolism of alanine was inhibited by aminooxyacetate, and the metabolic uncoupler dinitrophenol selectively accelerated the efflux of transported large neutral amino acids and L-lysine. Concurrent autoradiographic experiments suggest that transport sites for small and large neutral amino acids are localized in the basolateral plasma membrane of acinar, demilunar, and striated ductal cells.


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 Am. J. Physiol. Endocrinol. Metab.Home page
P. D. Watson
Analysis of the paired-tracer method of determining cell uptake
Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E366 - E371.
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