Journal of Dental Research, Vol 66, 1673-1679, Copyright © 1987 by International & American Associations for Dental Research Online Journals
Regional lymph node metastasis created by partial excision of carcinomas induced in hamster cheek pouch with 9,10-dimethyl-1,2-benzanthracene
T. Kage, M. Mogi, Y. Katsumata and T. Chino
Department of Oral & Maxillofacial Surgery I, Matsumoto Dental College, Nagano, Japan.
The feasibility of using the hamster cheek pouch/dimethyl-benzanthracene
(DMBA) system as an experimental model of lymphatic metastasis was
investigated. Forty male Syrian golden hamsters treated with DMBA were
divided into two equal groups--one with surgical excision of their tumors
and a control group without tumor excision. In the excision group, the
animals received three applications/week to the left cheek pouch of 0.3%
DMBA in acetone for 14 weeks. Following a three-week observation period,
the tumors in the pouch were excised at their base, and the animals were
killed after four weeks of further observation. In the control group, the
animals were treated for 14 weeks in a manner similar to that used for the
excision group, left for seven weeks without treatment, and then killed.
Cheek pouches with tumors and cervical lymph nodes were processed for
histological examination. All of the animals, both with and without
metastasis, had borne squamous cell carcinomas (SCC) in their treated cheek
pouches. Histologically, seven out of 16 animals in the excision group
showed metastatic deposits of SCC confined to the left cervical lymph
nodes, while in the control group, metastasis was not found in any of the
19 animals with SCC in their cheek pouches. The results demonstrate that
surgical excision of the hamster cheek pouch carcinoma is efficient in
producing unequivocal lymph node metastasis.
