Effect of NaF on Rat Parotid Gland Amylase Activity and cAMP Concentration in vitro and in vivo

¹ Department of Biochemistry, Indiana University School of Medicine, Indianapolis, Indiana 46223

The effect of NaF on the cAMP concentration in and amylase secretion from rat parotid glands in vitro and in vivo was investigated. In vitro, NaF (0.05 to 10 mmol/l) was found to increase significantly amylase secretion and cAMP concentration in parotid gland slices. In vivo, male rats injected intraperitoneally with 15 mg F/kg body weight as NaF had a significantly lower glandular amylase activity and higher plasma amylase activity than did the control rats injected with 0.9% NaCl. The fluoride concentration both in the parotid gland and plasma was highest at 30 min after injection and decreased with time in the fluoride-injected group. The concentration of the parotid gland cAMP in the fluoride-injected group was significantly higher than that in the control group. On the basis of these results, it is suggested that NaF, both in vitro and in vivo, increases the cAMP concentration, which subsequently stimulates amylase secretion from rat parotid glands.