JDR JDR Most Cited Articles
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Abiko, Y.
Right arrow Articles by Takiguchi, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abiko, Y.
Right arrow Articles by Takiguchi, H.

Journal of Dental Research, Vol 64, 106-111, Copyright © 1985 by International & American Associations for Dental Research Online Journals

ARTICLES

Glycylprolyl dipeptidylaminopeptidase from Bacteroides gingivalis

Y. Abiko, M. Hayakawa, S. Murai and H. Takiguchi

Dipeptidyl aminopeptidase activity was found in the culture medium of Bacteroides gingivalis 381. The enzyme, hydrolyzing glycylprolyl-4-methylcoumaryl-7-amide, was purified 750-fold from culture medium by ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DEAE Bio Gel A column chromatography. The molecular weight, determined by gel filtration, was approximately 160,000. The isoelectric point of the enzyme, estimated by isoelectric focusing using polyacrylamide disk gel electrophoresis, was about pH 6.2. The optimum pH of the enzyme was about 8.0, and the Km value was 0.05 mM. The enzyme activity was strongly inhibited by phenylmethylsulfonylfluoride and diisopropylfluorophosphate. The purified enzyme specifically cleaved glycylprolyl dipeptide from partially digested type I collagen.


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
Y. Kumagai, H. Yagishita, A. Yajima, T. Okamoto, and K. Konishi
Molecular Mechanism for Connective Tissue Destruction by Dipeptidyl Aminopeptidase IV Produced by the Periodontal Pathogen Porphyromonas gingivalis
Infect. Immun., May 1, 2005; 73(5): 2655 - 2664.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M.-C. Jobin, G. Martinez, J. Motard, M. Gottschalk, and D. Grenier
Cloning, Purification, and Enzymatic Properties of Dipeptidyl Peptidase IV from the Swine Pathogen Streptococcus suis
J. Bacteriol., January 15, 2005; 187(2): 795 - 799.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Banbula, M. Bugno, J. Goldstein, J. Yen, D. Nelson, J. Travis, and J. Potempa
Emerging Family of Proline-Specific Peptidases of Porphyromonas gingivalis: Purification and Characterization of Serine Dipeptidyl Peptidase, a Structural and Functional Homologue of Mammalian Prolyl Dipeptidyl Peptidase IV
Infect. Immun., March 1, 2000; 68(3): 1176 - 1182.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
Y. Kumagai, K. Konishi, T. Gomi, H. Yagishita, A. Yajima, and M. Yoshikawa
Enzymatic Properties of Dipeptidyl Aminopeptidase IV Produced by the Periodontal Pathogen Porphyromonas gingivalis and Its Participation in Virulence
Infect. Immun., February 1, 2000; 68(2): 716 - 724.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Banbula, P. Mak, M. Bugno, J. Silberring, A. Dubin, D. Nelson, J. Travis, and J. Potempa
Prolyl Tripeptidyl Peptidase from Porphyromonas gingivalis. A NOVEL ENZYME WITH POSSIBLE PATHOLOGICAL IMPLICATIONS FOR THE DEVELOPMENT OF PERIODONTITIS
J. Biol. Chem., April 2, 1999; 274(14): 9246 - 9252.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
IADR Journals Advances in Dental Research ®
Journal of Dental Research ® Critical Reviews (1990-2004)
Copyright © 1985 Institutional Access Guidelines